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The area of the axon (A) and myelin sheath (MS) of sciatic nerves was used to calculate the ratio of axon to myelin sheath area (A/MS)

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After 2 days, the medium was replaced with DMEM supplemented with 1 mg ml 1 insulin in 10% FBS and P/S (day 2), and it was changed every 2 days from day 4 to 8

[15] If a scheduled injection is missed, the patient should administer the dose as soon as remembered unless it is within 24 hours of the next scheduled dose, in which case the missed dose is omitted to prevent overlap